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| Registros recuperados : 8 | |
| 2. |  | NEGRISOLI JUNIOR, A. S.; BALDANI, J. I.; SA, M. F. G. de; SILVA, M. C. M. da; MACEDO, L. L. P. de; FONSECA, F. C. de A.; NEGRISOLI, C. R. de C. B.; GUZZO, E. C. Manejo da broca-gigante da cana-de-açúcar (Telchinlicus) (Drury) (Lepidoptera: Castniidae) no nordeste do Brasil. Aracaju: Embrapa Tabuleiros Costeiros, 2015. 52 p. (Embrapa Tabuleiros Costeiros. Documentos, 198).| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Tabuleiros Costeiros. |
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| 3. | | REGO, E. C. S.; PINHEIRO, T. D. M.; FONSECA, F. C. de A.; GOMES, T. G.; COSTA, E. de C.; BASTOS, L. S.; ALVES, G. S. C.; COTTA, M. G.; AMORIM, E. P.; FERREIRA, C. F.; TOGAWA, R. C.; COSTA, M. M. do C.; GRYNBERG, P.; MILLER, R. N. G. Characterization of microRNAs and Target Genes in Musa acuminata subsp. burmannicoides, var. Calcutta 4 during Interaction with Pseudocercospora musae. Plants, v. 12, 1473, 2023.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 4. | | FIRMINO, A. A. P.; PINHEIRO, D. H.; MOREIRA-PINTO, C. E.; ANTONINO, J. D.; MACEDO, L. L. P.; MARTINS-DE-SA, D.; ARRAES, F. B. M.; COELHO, R. R.; FONSECA, F. C. de A.; SILVA, M. C. M.; ENGLER, J. de A.; SILVA, M. S.; LOURENÇO-TESSUTTI, I. T.; TERRA, W. R.; GROSSI-DE-SA, M. F. RNAi-mediated suppression of Laccase2 impairs cuticle tanning and molting in the cotton boll weevil (Anthonomus grandis). Frontiers in Physiology, v. 11, article 591569, 2020.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 5. | | FONSECA, F. C. de A.; FIRMINO, A. A. P.; MACEDO. L. L. P. de; COELHO, R. R.; SOUSA JÚNIOR, J. D. A. de; SILVA JUNIOR, O. B.; TOGAWA, R. C.; PAPPAS JUNIOR, G. J.; GÓIS, L. A. B. de; SILVA, M. C. M. da; SA, M. F. G. de. Sugarcane giant borer transcriptome analysis and identification of genes related to digestion. Plos One, fev., 2015.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 6. | | FIRMINO, A. A. P.; FONSECA, F. C. de A.; MACEDO, L. L. P. de; COELHO, R. R.; SOUZA JÚNIOR, J. D. A. de; TOGAWA, R. C.; SILVA JUNIOR, O. B. da; PAPPAS JÚNIOR, G. J.; SILVA, M. C. M. da; ENGLER, G.; GROSI DE SÁ, M. F. Transcriptome analysis in cotton Boll Weevil (Anthonomus grandis) and RNA interference in insect pests. Plos One, v. 8, n. 12, e85079, 2013.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 7. | | PINHEIRO, T. D. M.; REGO, E. C. S.; ALVES, G. S. C.; FONSECA, F. C. de A.; COTTA, M. G.; ANTONINO, J. D.; GOMES, T. G.; AMORIM, E. P.; FERREIRA, C. F.; COSTA, M. M. do C.; GRYNBERG, P.; TOGAWA, R. C.; MILLER, R. N. G. Transcriptome profiling of the resistance response of Musa acuminata subsp. burmannicoides, var. Calcutta 4 to Pseudocercospora musae. International Journal of Molecular Sciences, v. 23, 2022. 13589.| Biblioteca(s): Embrapa Mandioca e Fruticultura; Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | FONSECA, F. C. de A.; ANTONINO, J. D.; MOURA, S. M. de; RODRIGUES-SILVA, P. L.; MACEDO, L. L. P. de; GOMES JÚNIOR, J. E.; LOURENCO, I. T.; LUCENA, W. A.; MORGANTE, C. V.; RIBEIRO, T. P.; MONNERAT, R. G.; RODRIGUES, M. A.; CUCCOVIA, I. M.; SILVA, M. C. M. da; SA, M. F. G. de. In vivo and in silico comparison analyses of Cry toxin activities toward the sugarcane giant borer. Bulletin of Entomological Research, v. 113, n. 3, p. 335-346, 2023. Na publicação: Leonardo Lima Pepino Macedo; Isabela Tristan Lourenço-Tessuti; Carolina Viana Morgante; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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| Registros recuperados : 8 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
26/05/2023 |
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Data da última atualização: |
29/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
FONSECA, F. C. de A.; ANTONINO, J. D.; MOURA, S. M. de; RODRIGUES-SILVA, P. L.; MACEDO, L. L. P. de; GOMES JÚNIOR, J. E.; LOURENCO, I. T.; LUCENA, W. A.; MORGANTE, C. V.; RIBEIRO, T. P.; MONNERAT, R. G.; RODRIGUES, M. A.; CUCCOVIA, I. M.; SILVA, M. C. M. da; SA, M. F. G. de. |
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Afiliação: |
FERNANDO CAMPOS DE ASSIS FONSECA, University of Brasília; JOSÉ DIJAIR ANTONINO, University of Brasília; STÉFANIE MENEZES DE MOURA, National Institute of Science and Technology; PAOLO LUCAS RODRIGUES-SILVA, National Institute of Science and Technology; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; JOSÉ EDÍLSON GOMES JÚNIOR, Federal University of Brasília; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; WAGNER ALEXANDRE LUCENA, ARIG; CAROLINA VIANNA MORGANTE, CPATSA; THUANNE PIRES RIBEIRO, National Institute of Science and Technology; ROSE GOMES MONNERAT; MAGALI APARECIDA RODRIGUES, 7University of São Paulo; IOLANDA MIDEA CUCCOVIA, University of São Paulo; MARIA CRISTINA MATTAR DA SILVA, Cenargen; MARIA FATIMA GROSSI DE SA, Cenargen. |
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Título: |
In vivo and in silico comparison analyses of Cry toxin activities toward the sugarcane giant borer. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Bulletin of Entomological Research, v. 113, n. 3, p. 335-346, 2023. |
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DOI: |
https://doi.org/10.1017/S000748532200061X |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Leonardo Lima Pepino Macedo; Isabela Tristan Lourenço-Tessuti; Carolina Viana Morgante; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa. |
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Conteúdo: |
The sugarcane giant borer, Telchin licus licus, is an insect pest that causes significant losses in sugarcane crops and in the sugar-alcohol sector. Chemical and manual control methods are not effective. As an alternative, in the current study, we have screened Bacillus thuringiensis (Bt) Cry toxins with high toxicity against this insect. Bioassays were conducted to determine the activity of four Cry toxins (Cry1A (a, b, and c) and Cry2Aa) against neonate T. licus licus larvae. Notably, the Cry1A family toxins had the lowest LC50 values, in which Cry1Ac presented 2.1-fold higher activity than Cry1Aa, 1.7-fold larger than Cry1Ab, and 9.7-fold larger than Cry2Aa toxins. In silico analyses were performed as a perspective to understand putative interactions between T. licus licus receptors and Cry1A toxins. The molecular dynamics and docking analyses for three putative aminopeptidase N (APN) receptors (TlAPN1, TlAPN3, and TlAPN4) revealed evidence for the amino acids that may be involved in the toxin–receptor interactions. Notably, the properties of Cry1Ac point to an interaction site that increases the toxin's affinity for the receptor and likely potentiate toxicity. The interacting amino acid residues predicted for Cry1Ac in this work are probably those shared by the other Cry1A toxins for the same region of APNs. Thus, the presented data extend the existing knowledge of the effects of Cry toxins on T. licus licus and should be considered in further development of transgenic sugarcane plants resistant to this major occurring insect pest in sugarcane fields. MenosThe sugarcane giant borer, Telchin licus licus, is an insect pest that causes significant losses in sugarcane crops and in the sugar-alcohol sector. Chemical and manual control methods are not effective. As an alternative, in the current study, we have screened Bacillus thuringiensis (Bt) Cry toxins with high toxicity against this insect. Bioassays were conducted to determine the activity of four Cry toxins (Cry1A (a, b, and c) and Cry2Aa) against neonate T. licus licus larvae. Notably, the Cry1A family toxins had the lowest LC50 values, in which Cry1Ac presented 2.1-fold higher activity than Cry1Aa, 1.7-fold larger than Cry1Ab, and 9.7-fold larger than Cry2Aa toxins. In silico analyses were performed as a perspective to understand putative interactions between T. licus licus receptors and Cry1A toxins. The molecular dynamics and docking analyses for three putative aminopeptidase N (APN) receptors (TlAPN1, TlAPN3, and TlAPN4) revealed evidence for the amino acids that may be involved in the toxin–receptor interactions. Notably, the properties of Cry1Ac point to an interaction site that increases the toxin's affinity for the receptor and likely potentiate toxicity. The interacting amino acid residues predicted for Cry1Ac in this work are probably those shared by the other Cry1A toxins for the same region of APNs. Thus, the presented data extend the existing knowledge of the effects of Cry toxins on T. licus licus and should be considered in further development of transgenic s... Mostrar Tudo |
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Palavras-Chave: |
Aminopeptidase N; Broca gigante da cana-de-açúcar; Cry toxins; Modelagem molecular; Molecular modeling; Techin licus licus; Toxinas Cry. |
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Thesagro: |
Bacillus Thuringiensis; Cana de Açúcar; Inseto; Praga; Saccharum Officinarum. |
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Thesaurus NAL: |
Sugarcane. |
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Categoria do assunto: |
--
O Insetos e Entomologia |
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Marc: |
LEADER 03134naa a2200469 a 4500
001 2154056
005 2023-05-29
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1017/S000748532200061X$2DOI
100 1 $aFONSECA, F. C. de A.
245 $aIn vivo and in silico comparison analyses of Cry toxin activities toward the sugarcane giant borer.$h[electronic resource]
260 $c2023
500 $aNa publicação: Leonardo Lima Pepino Macedo; Isabela Tristan Lourenço-Tessuti; Carolina Viana Morgante; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa.
520 $aThe sugarcane giant borer, Telchin licus licus, is an insect pest that causes significant losses in sugarcane crops and in the sugar-alcohol sector. Chemical and manual control methods are not effective. As an alternative, in the current study, we have screened Bacillus thuringiensis (Bt) Cry toxins with high toxicity against this insect. Bioassays were conducted to determine the activity of four Cry toxins (Cry1A (a, b, and c) and Cry2Aa) against neonate T. licus licus larvae. Notably, the Cry1A family toxins had the lowest LC50 values, in which Cry1Ac presented 2.1-fold higher activity than Cry1Aa, 1.7-fold larger than Cry1Ab, and 9.7-fold larger than Cry2Aa toxins. In silico analyses were performed as a perspective to understand putative interactions between T. licus licus receptors and Cry1A toxins. The molecular dynamics and docking analyses for three putative aminopeptidase N (APN) receptors (TlAPN1, TlAPN3, and TlAPN4) revealed evidence for the amino acids that may be involved in the toxin–receptor interactions. Notably, the properties of Cry1Ac point to an interaction site that increases the toxin's affinity for the receptor and likely potentiate toxicity. The interacting amino acid residues predicted for Cry1Ac in this work are probably those shared by the other Cry1A toxins for the same region of APNs. Thus, the presented data extend the existing knowledge of the effects of Cry toxins on T. licus licus and should be considered in further development of transgenic sugarcane plants resistant to this major occurring insect pest in sugarcane fields.
650 $aSugarcane
650 $aBacillus Thuringiensis
650 $aCana de Açúcar
650 $aInseto
650 $aPraga
650 $aSaccharum Officinarum
653 $aAminopeptidase N
653 $aBroca gigante da cana-de-açúcar
653 $aCry toxins
653 $aModelagem molecular
653 $aMolecular modeling
653 $aTechin licus licus
653 $aToxinas Cry
700 1 $aANTONINO, J. D.
700 1 $aMOURA, S. M. de
700 1 $aRODRIGUES-SILVA, P. L.
700 1 $aMACEDO, L. L. P. de
700 1 $aGOMES JÚNIOR, J. E.
700 1 $aLOURENCO, I. T.
700 1 $aLUCENA, W. A.
700 1 $aMORGANTE, C. V.
700 1 $aRIBEIRO, T. P.
700 1 $aMONNERAT, R. G.
700 1 $aRODRIGUES, M. A.
700 1 $aCUCCOVIA, I. M.
700 1 $aSILVA, M. C. M. da
700 1 $aSA, M. F. G. de
773 $tBulletin of Entomological Research$gv. 113, n. 3, p. 335-346, 2023.
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